Online laboratory continuing education for clinical laboratories and med techs. Oxidize in 0.
Rinse in distilled water. A good Schiff reagent will rapidly turn a red-purple color.
Solutions and Reagents: Nice and educative Reply. All Rights Reserved.
Differentiation can be accomplished by: Add sodium metabisulphite and HCl. Warm to hot running water develops the PAS stain and gives a more intense staining reaction than cold water. A deteriorating schiff reagent will give a delayed reaction and the color produced will be a deep blue-purple. Dehydrate and coverslip using a synthetic mounting medium. A suitable basic stain is used as counter stain.
Add charcoal, shake for one minute and filter.
Highly chlorinated water can cause these excess molecules to nonspecifically stain other tissue elements in the section. Sign up for LabCE's mailing list.
Name required. While some technicians omit this reagent step in the protocol with no problems, metabisulfite rinses may be necessary to remove any excess leucofuchsin left over after exposure to the Schiff reagent.
Gluteraldehyde should be avoided as a tissue fixative since it is a dialdehyde that will react with this reagent and give false-positive PAS staining.
Periodic Acid 0. Post Staining Procedure: