What is the pas procedure

what is the pas procedure

Online laboratory continuing education for clinical laboratories and med techs. Oxidize in 0.

Rinse in distilled water. A good Schiff reagent will rapidly turn a red-purple color.

what is the pas procedure

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what is the pas procedure

Differentiation can be accomplished by: Add sodium metabisulphite and HCl. Warm to hot running water develops the PAS stain and gives a more intense staining reaction than cold water. A deteriorating schiff reagent will give a delayed reaction and the color produced will be a deep blue-purple. Dehydrate and coverslip using a synthetic mounting medium. A suitable basic stain is used as counter stain.

Add charcoal, shake for one minute and filter.

what is the pas procedure

Highly chlorinated water can cause these excess molecules to nonspecifically stain other tissue elements in the section. Sign up for LabCE's mailing list.

Periodic Acid Schiff (PAS) Staining Technique For Carbohydrates

Name required. While some technicians omit this reagent step in the protocol with no problems, metabisulfite rinses may be necessary to remove any excess leucofuchsin left over after exposure to the Schiff reagent.

what is the pas procedure

Gluteraldehyde should be avoided as a tissue fixative since it is a dialdehyde that will react with this reagent and give false-positive PAS staining.

Periodic Acid 0. Post Staining Procedure: