Whole genome shotgun sequencing ppt third

Are "shotgun cloning" and "clone based sequencing" actually mutually exclusive terms?

whole genome shotgun sequencing ppt third

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whole genome shotgun sequencing ppt third

The v short answer is that in shotgun sequencing, the sequencer is fed a set of random sequences from the target. Celera were competing with Sanger and collaborators to sequence the human genome.

whole genome shotgun sequencing ppt third

What's the difference between shotgun sequencing and clone based sequencing? Email Required, but never shown.

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Ask Question. Like that, data management and the mounting of DNA contigs will be a lot easier computationally speaking. BACs can be 150 kb long just to give you an idea. Biology Stack Exchange works best with JavaScript enabled.

Post Your Answer Discard By clicking "Post Your Answer", you acknowledge that you have read our updated terms of service , privacy policy and cookie policy , and that your continued use of the website is subject to these policies. To me it seems like both clone based sequencing and shotgun sequencing could have been used together, however the lecturers semantics were a bit vague...

whole genome shotgun sequencing ppt third

I think you better mean whole genome shotgun sequencing with "shotgun sequencing" as the clone based sequencing also is a shotgun sequencing method. In clone based sequencing, the DNA to be sequenced is specifically sectioned off into sequence copied into of plasmids BACs - bacterial artificial chromosome to divide the sequencing job into sections- a divide and conquer strategy.

The public project cut up the genome into BACs and shotgunned every BAC, and then put the BACs together, while Venter used some sequence position information from the public effort to help him place his shotgun pieces.

whole genome shotgun sequencing ppt third

These are not mutually exclusive terms ; clone-based sequencing projects usually use the large clone libraries to section the project into a set of shotgun sequencing projects. Celera knew they couldn't compete with Sanger et al. By using our site, you acknowledge that you have read and understand our Cookie Policy , Privacy Policy , and our Terms of Service.