For quantification of glucose in reaction mixtures, calculate the areas of the target peak obtained by ion chromatography by the Chromeleon software, and then determine the glucose concentration using the standard curve of the glucose standards Figure 2. Monitor the growth of cyanobacterial cells by measuring the optical density OD at 730 nm with a spectrophotometer. Planta 228 4: Here, we report our recent IC-based protocol for determination of the invertase activity in cyanobacterial cells.
Whereas, the protein concentrations of crude extracts are 2. Sucrose synthase is involved in the conversion of sucrose to polysaccharides in filamentous nitrogen-fixing cyanobacteria.
Tubes can be boiled together within 15 minutes of stopping reaction. In fact, most of the invertase enzymatic activity assays are based on the detection of the generated glucose product.
Procedure 7. For each 10 sec vortexing, cool the tube on ice for 5 sec. Vol 8, Iss 10, May 20, 2018.
All the methods for determination of the invertase activity are dependent on detection of the glucose product generated by the invertase. Invertase EC 3.
Scope This procedure applies to all products that have specification for the enzymatic activity of Invertase at Sigma-Aldrich Saint Louis. Spin down the reaction mixtures at 12,000 x g for 10 sec. Origin of sucrose hydrolysis in the plant cytosol?
Lu , the Shandong Taishan Scholarship X. Photosynthesis driven conversion of carbon dioxide to fatty alcohols and hydrocarbons in cyanobacteria.
Planta 216 6: Invertase can catalyze the hydrolysis of sucrose, and is widely distributed in cells of cyanobacteria and plants. Culture volume should be less than 30 ml in 50 ml flasks. Reproduction of any materials from the site is strictly forbidden without permission.