Heart regeneration in zebrafish. Acta Ophthalmol. When analyzing non-normal datasets, Mann-Whitney U test was utilized to determine the significance of differences between unablated and ablated larvae.
In conclusion, a single weight-adjusted dose of rNRG1 induces transient increases of serum concentration in mice. MRI, visual inspection, and histopathological analyses showed no neoplastic growth.
The lowest reported concentration of rNRG1 in serum was 0.
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Mizota A, Adachi-Usami E. Due to its importance in maintaining retinal function, diseases affecting the RPE have dire consequences for vision. This offers one possible explanation for the lack of general growth effects.
A video depicting each optical slice of an ablated 3dpi larvae. Administration of rNRG1 does not induce neoplastic growth ErbB2 gene amplification in cancer causes constitutive signaling. Response of the retinal pigment epithelium to selective photocoagulation.
The measured RPE was divided into quintiles cartoon , and the area under the curve within each quintile was measured. Some NRG forms can be released from the cell surface by cleavage through metalloproteinases[ 14 — 16 ].
Considering the cellular targets of rNRG1 could provide additional explanations for the lack of unwanted growth effects. Santoro F, Sahara M. Thus, we hypothesized that uninjured peripheral RPE cells respond to injury by dedifferentiating and proliferating to replace lost tissue. In contrast, the biochemical mechanisms leading to over-activation of cellular proliferation in cancer are fundamentally different. Neural retinal regeneration in the anuran amphibian Xenopus laevis post-metamorphosis: H,I Larvae exposed to BrdU 5-6dpi and fixed at 7dpi.
The present study complements our prior presentation of a therapeutic strategy for rNRG1 administration in pediatric patients[ 13 ]. Retinal pigment epithelium damage enhances expression of chemoattractants and migration of bone marrow-derived stem cells. Our zebrafish RPE ablation model differs significantly from Xenopus [ 87 ], newt [ 88 , 89 ], and embryonic chick [ 90 — 92 ] retinectomy models wherein the entire retina is surgically removed and subsequently regenerates from remaining RPE tissue that that transdifferentiates, proliferates, and regenerates retinal tissue.Ninth Doctor Regenerates - Christopher Eccleston to David Tennant - Doctor Who - BBC
Further animal and human studies are required to establish a comprehensive safety profile. In mammals, RPE regeneration is limited and dependent upon the size of the injury [ 24 ]; small lesions can be repaired by the expansion of adjacent RPE [ 25 , 26 ], but existing RPE are unable to repair large lesions [ 24 , 27 — 30 ].