Published 25 February 2015. Non-genetic heterogeneity of cells in development: Additionally, the small size of zebrafish embryos and larvae make them ideal for phenotypic chemical screening. Zebrafish Maintenance and Husbandry.
Some protocols require a few additional steps for embryo maintenance. Eales JG 1979 Thyroid functions in cyclostomes and fishes.
Download references. The spots were semi-automatically counted using the previously published program written in MATLAB Mathworks 32 with the following modifications.
Individual cells are indicated by white enclosing lines. Little, S.
Expression analysis of two different genes using whole-mount smFISH protocol. Nagasaka A, Hidaka H 1976 Effect of antithyroid agents 6-propyl-2-thiouracil and 1-mehtyl-2-mercaptoimidazole on human thyroid iodine peroxidase.
These steps were repeated for each cell. Horm Evol 1: Correspondence to Thomas N. In order to obtain data on gene expression noise in individual cells with spatial resolution but without preparing hundreds of sections, a protocol applicable to a whole-mount vertebrate animal is desirable. Williams 1 , Morgan E. Finally, the video concludes with some practical applications of these techniques in developmental research.
This smFISH method together with single cell qRT-PCR or RNA-seq analyses with sorted cells from zebrafish embryo provides a quantitative tool for unveiling regulatory mechanism for developmental, physiological and disease processes with the single cell resolution. An Introduction to the Chick: Essential role of lysyl oxidases in notochord development.
Sugimura, T. Embryos were dechorionated with tweezers if they were not hatched prior to fixation. ALTEX 31 3: